Fragment Analysis
Project Considerations
FADSS provides analysis of PCR-based amplification products.
FADSS can accommodate a wide array of dye tags and size standards. Please contact FADSS staff to discuss your specific project details.
Applications
- Microsatellite analysis
- AFLP
- TRFLP
- BAC fingerprinting
- SNP genotyping
- LOH analysis (loss of heterozygosity)
- SNapSHot analysis
Sample Submission Guidelines
1. Please provide at least 4ul of sample at the appropriate concentration in water or Tris (not TE!)
2. Please submit samples in 8-well strip tubes or in plates. Place clearly labeled samples on the top shelf of the freezer in SCI355. The plate and or rack label should match the Plate/Rack ID. If you are shipping your samples to FADSS, please send your samples to the address at the top of the DNA Fragment Analysis Request form Attn: FADSS.
3. Please submit the completed Fragment Analysis Request Form to fadss.ok@ubc.ca. Incomplete request forms may result in delayed processing.
Fragment Analysis Service Fees
| Number of Samples* | Price** |
|---|---|
| Less than 1/2 plate (1-47 wells) | $4.00 |
| 1/2 plate-full plate(48-95 wells) | $3.50 |
| Full plate (96 wells) | $3.00 |
*Please note, samples are charged in multiples of 8.
**Project and optimization rates may be available. Please contact FADSS for more details.
The user may request a rerun of samples with poor results. If subsequent results do not improve, the user will be charged the <1/2 plate Fragment Analysis rate. If subsequent results improve, the user will not be charged for the reruns.
Turn-around Time
Typical turnaround time is 1-2 business days upon receipt of sample submission. Please allow more time for large submissions (multiple plates).
Accessing Fragment Analysis Results
Your UBC O clients can obtain their fragment analysis results on and off campus by logging into CWL. The FASTA files (.fsa) will be located in the following directory:
K:\RAA\FADSS\username
Non-UBC O clients can obtain their results at https://payforprint.ok.ubc.ca/netstorage/ using a Netstorage account and password assigned by FADSS.
Analyzing Fragment Analysis Results
The .fsa FASTA files generated can be used with a variety of software programs. FADSS facilities include computers with Applied Biosystems’ GeneMapper v4.0 software. For clients who do not have access to our facilities, Applied Biosystems offers Peak Scanner, a freeware software program.
Last reviewed 
7/24/2012 2:50:55 PM
